Expression of apoptosis assotiated gene BCL-2,BAX and the relationship between expression and apoptosis in non-small-cell lung cancer

Objective To identify the effect of Bcl-2/Bax on the mechanism of NSCLC and to detect two kinds of protein and apoptosis in NSCLC in the experiment.Methods 48 cases of NSCLC after operation in the near future and 15 cases of normal tissue were chosen randomly in this experiment.Bcl-2 and Bax were detected by immunohistochemistry SP technique,and the apoptosis was detected in TUNEL.Results Bcl-2,Bax,AI were all expressed higher in NSCLC than those in normal lung tissues(P0.05).The positive points of Bcl-2,Bax,AI in differentiation grade,TNM stage,lymphatic metastasis had statistical significance(P0.05).The relationship between Bcl-2 positive points and apoptosis indexes was negatively correlated(r=-0.609,P0.05),but it was positively correlated between Bax positive points and apoptosis indexs(r=0.821,P0.05).Conclusion In this experiment,Bcl-2 has participated in the pristine genesis and development and has inhibited the apoptosis of NSCLC.Bax is concerned with the development,metastasis and the increase of apoptosis level in the progression of NSCLC.