Amplification of Human Epidermal Cells by a Rotary Bioreactor in Vitro

Objective: We attempted to produce human epidermal cells(hECs) on a large scale by Rotary Cell Culture System(RCCS).In this cultural system we used Cytodex-3 microbeads as microcarriers and high aspect ratio vessel RCCS as cultural system.Methods:The hECs were obtained from human skin by using the method of digesting human foreskin with Dispase II and Trypsin-EDT and cultured in vitro and labeled with DIL,then combined with Cytodex-3 in RCCS.The characteristics of hECs combined with microcarriers were observed with Inverted microscope,and scanning electronic microscope(SEM).The proliferative capacities of microgravity cultivation and routine cultivation were compared by group doubling time.Results:Our results showed that hECs rapidly attached to the microcarriers in the RCCS,achieving high cell densities.hECs cultured on the microbeads in the RCCS remained actively proliferating potentials.Conclusion:Using biological reactor and microcarriers culture human epidermal cells is a kind of effective method for preparation a large number of seed cells for tissue engineering.