Rapidly large scale proliferation of liver cell line by microcarrier vehicle in rotary cell culture system

Objective To evaluate the feasibility of large scale culture of liver cell by microcarrier vehicle in rotary cell culture system(RCCS).Methods Liver cell line CL-1 and HepFMMU were rapidly proliferated by microcarrier vehicle in RCCS in a short period of time.Histomorphology and biological functions were evaluated accordingly in phase contrast observation,scanning electron microscope(SEM),biological functions test and MTT test.Results Liver cell cultivated in the bioreactor in three dimensions were expanded rapidly with shorter doubling time.The cell population could reach 23 times more than that before expansion in fifth day and decreased afterward.The expanded cells performed well in major functions examined by inverted microscope in phase contrast observation,scanning electron microscope(SEM),biological functions test and MTT test.Conclusion It is feasible for rapidly large scale amplification for liver cell by microcarrier vehicle in RCCS.But further research is needed to improve the supply of energy and oxygen and enhance the clearness of waste of RCCS though better simulating the liver structure due to high demand of liver cell density.