Comparison of different methods of serological testing for syphilis and its clinical value

Objective To explore three different serological detection of syphilis in syphilis diagnosis and treatment of clinical value.Methods From 2008 to 2010,6 614 samples collected in transfusion department and the STD of our hospital were tested by enzyme-linked immunosorbent assay(ELISA) and treponema pallidum particle assay(TPPA).Then the positive samples were detected by toluidine red unheated serum test(TRUST) and tested the titer.Results ELISA method detected 247 positive samples,TPPA method detected 244 positive samples,and the coincidence rate of positive samples tested by TPPA method ELISA was 98.78%,the detection rate between the two was not significantly different(P0.05);136 out of the positive samples tested by ELISA were positve by TRUST method,with positive coincidence rate of 55.06%;136 out of the positive samples tested by TPPA were positve by TRUST method,with positive coincidence rate of 55.74%.There were significant differences of detection rate among TRUST method,ELISA method and the TPPA method(P0.05).Conclusion There are many methods to detect syphilis,and the sensitivity and specificity of each method are not the same,through the comparative analysis of three detection methods,the diagnosis and treatment of syphilis should be combined with different methods of syphilis detection.