Analysis of Genetic Diversity in Leek Cultivars Based on RAPD

Random amplified polymorphic DNA(RAPD) markers were used to estimate the genetic diversity in 15 cultivars of Allium tuberosum(leek) from 11 regions in China.Twenty random primers generated a total of 136 reproducible and discernible loci,among which 98 were polymorphic with the percent of polymorphic loci of 72%.Based on the Nei's genetic distances between the 15 cultivars,UPGMA cluster analysis was performed and showed that all cultivars were classified into four groups at genetic distance of 0.34,i.e.,Goup 1 contained 3 cultivars(Du Genhong,Lanzhou baigen and Nanjing mabianjiu);Goup 2 included 3 cultivars(Hanzhong dongjiu,Shanghai qiangjiu-3 and Shanghai qiangjiu-6);Goup 3 contained 5 cultivars(Sijiqing,Guilin xiaohonggen,Tianjin daqingmiao,Xinjiang baigen and Jiaxing xuelian);Goup 4 included 4 cultivars(Tianjin dajingou,Huanjiu,Pingfeng taijiu and Kanghan taijiu).There were no correlation between cultivars and origin regions of leeks.