RAPD analysis of genetic variation in different cauliflower cultivars
Huang Congli, LI Chuan-yong, Aimin Pan, Weidong Xu, Xu Ruide
Abstract
Huang Congli, LI Chuan-yong, Aimin Pan, Weidong Xu, Xu Ruide
Abstract
Genetic DNA diversity among thirteen cauliflower cultivars were analyzed using Random Amplified Polymorphic DNA (RAPD). There were 175 clear and reproducible bands amplified with twenty primers and 118 of these bands were polymarphic(67.4%). The genetic similarity coefficients between different cauliflower cultivars based on RAPD markers were analyzed and the dendrogram of different cultivars based on UPGMA analysis was also generated. The results showed that the genetic diversity among different cultivars was abundant. The genetic relationship among cultivars, application of RAPD technique in differentiation and breeding of cauliflower were preliminary discussed.
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Genetic DNA diversity among thirteen cauliflower cultivars were analyzed using Random Amplified Polymorphic DNA (RAPD). There were 175 clear and reproducible bands amplified with twenty primers and 118 of these bands were polymarphic(67.4%). The genetic similarity coefficients between different cauliflower cultivars based on RAPD markers were analyzed and the dendrogram of different cultivars based on UPGMA analysis was also generated. The results showed that the genetic diversity among different cultivars was abundant. The genetic relationship among cultivars, application of RAPD technique in differentiation and breeding of cauliflower were preliminary discussed.
Key concepts: RAPD, UPGMA, Dendrogram, Cultivar, Genetic diversity, Biology, Genetic variation, Botany