The Exploration of Changes of CD4~+CD25~+ Regulatory T Cells in Peripheral Blood of Patients with Non-Hodgkin's Lymphoma

Objective: To detect the proportion of CD4+CD25+ regulatory T cells(Treg) in peripheral blood of patients with non-Hodgkin's lymphoma(NHL),and to explore the relationship between Treg and NHL.Methods: By using flow cytometry with surface staining fluorochrome-conjugated antibodies for CD4,CD25,the percentages of CD4+CD25+Treg in peripheral blood of 60 patients with NHL and 60 Healthy controls were detected and analyzed.Results: ①The average peripheral blood CD4+ levels of patients with NHL before chemotherapy was significant lower than that of the control group(P0.05),while the percentages of CD4+CD25+Treg was significant higher than that of the control group(P0.05).②After chemotherapy,the percentages of CD4+CD25+Treg was decreased and CD4+ levels was increased than before chemotherapy.The difference between them was statistically significant(P0.05).③ There was no significant difference between the control group and patients with NHL after chemotherapy of peripheral blood CD4+ level(P 0.05).But the percentages of CD4+CD25+Treg of patients with NHL after chemotherapy was still higher than the control group(P0.05).Conclusion: The increase in Tregs in patients with NHL may induce immune suppression and chemotherapy may cause the decreasing of Tregs.