Optimization of Microwave-assisted Extraction of RNA from Waste Beer Yeast Cell Based on Design of Multiple Linear Regression

In order to develop and utilize waste beer yeast,this experiment was design to extract nucleic acid from waste beer yeast through microwave heating method. Effects of microwave power,microwave temperature,microwave treatment time,material to liquid ratio,extraction time,extraction temperature on extraction rate of nucleic acid were investigated. Through multiple linear regression test,the main extraction conditions were determined as follows:microwave power 700 W,microwave time 4 min,extraction time 3.5 h,and extraction temperature 100 ℃. The total acid content of obtained sample is 10.920 μg/ml. Compared with the traditional method,the microwave method is of shorter extraction time,higher efficiency and lower energy consumption,and can be applied in the industrial production.