Expression and identification of CsSCCRO gene of Clonorchis sinensis

Objective To transfect CsSCCRO gene to Hela cell,and detect transfection efficiency,so as to provide evidence for the encoding protein function research on the cellular level.Methods Cloned CsSCCRO gene to eukaryotic vector pEGFP-N1 and transfected the pEGFP-N1-CsSCCRO to Hela cell by the method of liposome,then identified the transfection efficiency by RT-PCR.Results Recombinant plasmid pEGFP-N1-CsSCCRO was successfully transfected to Hela cell,and fluorescin were expressed stably.A 780 bp length fragment was successfully cloned and identified by RT-PCR.Conclusion CsSCCRO gene can transfect to Hela cell and can be transcribed and translated to fusion protein with GFP efficiently.