The Construction of pEGFP-HPV16E6 Fusion Gene in Eukaryotic Expression Vector and its Expression in Hela Cells

Objective To construct pEGFP-HPV16 E6 fusion gene in eukaryotic expression vector and to investigate its expression in Hela cells.Methods HPV16 E6 ORF was amplified from PCR2.1-HPV16E6 by PCR and inserted into plasmid pEGFP-C2.Using lipofectin method,the recombinant expression plasmid pEGFP-HPV16 E6 was transfected into Hela cells.Conclusions The results showed that pEGFP-HPV16 E6 expressed a fusion protein with green fluorescence protein in Hela cells as defined by Western blot analysis.This fusion protein was localized in the nucleus of Hela cells as detected by fluorescence microscopy.