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2007•Journal of Tianjin University of Traditional Chinese MedicineRequires access

Study of fingerprint of rhizoma corydalis by HPLC

Min Zhang

Open publisher page 1 citations

Abstract

[Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi. The conclusion makes reference to standard of quality control of Rhizoma Corydalis. [Methods] The methods were performed by RP-HPLC. Kromasil ODS-C18 Column was used at 30 ℃, acetonitrile-acetate buffer solution (pH 6.0) as mobile phase, gradient eluting, flow rate was 1.0 mL/min, detection wavelength was set at 280 nm and eluting period were 75 min. [Results] HPLC-fingerprint of Rhizoma Corydalis was established, which contained 17 common peaks. [Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis.

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What this paper is about

[Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi. The conclusion makes reference to standard of quality control of Rhizoma Corydalis. [Methods] The methods were performed by RP-HPLC. Kromasil ODS-C18 Column was used at 30 ℃, acetonitrile-acetate buffer solution (pH 6.0) as mobile phase, gradient eluting, flow rate was 1.0 mL/min, detection wavelength was set at 280 nm and eluting period were 75 min. [Results] HPLC-fingerprint of Rhizoma Corydalis was established, which contained 17 common peaks. [Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis.

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Available abstract

[Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi. The conclusion makes reference to standard of quality control of Rhizoma Corydalis. [Methods] The methods were performed by RP-HPLC. Kromasil ODS-C18 Column was used at 30 ℃, acetonitrile-acetate buffer solution (pH 6.0) as mobile phase, gradient eluting, flow rate was 1.0 mL/min, detection wavelength was set at 280 nm and eluting period were 75 min. [Results] HPLC-fingerprint of Rhizoma Corydalis was established, which contained 17 common peaks. [Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis.

Key concepts: Corydalis, Chromatography, High-performance liquid chromatography, Fingerprint (computing), Chemistry, Tetrahydropalmatine, Medicine, Computer science

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