Solid fermentation straw of cellulase produced by Penicillium glaucum

With wheat bran and wheat straw as raw materials,optimum medium and fermentation conditions which solid fermentation of cellulase produced by Penicillium glaucum was optimized by orthogonal and single-factor test.The cellulose content of wheat straw was compared before and after fermentation.The result showed that the optimal medium were as follows:wheat straw∶wheat braw was 3∶2,nitrogen source was(NH4)3PO4,water content 200 %,pH 4.5.The optimum fermentation conditions were as follows:cultivation time 72 h,temperature 40 ℃,pH 5.0,nitrogen content 0.6 %,inoculum 20 % and semi-enclosed cultivation.The NDF,ADF,cellulose and hemi-cellulose content of wheat straw after fermentation dropped by 4.29 %,3.89 %,4.66 % and 5.82 % than before the fermentation,no significant change was found in lignin.