Study on the Clone of Human Adiponectin Gene and Its Expression in Escherichia Coli

Objective: To clone human adiponectin gene and identify its expression in E.coli. Methods: Total RNA was extracted from human subcutaneous adipose tissue and a full length cDNA was obtained by RT-PCR. The product was ligated to pMD-18T vector, and was sequenced and constructed to the expression vector in pET-DEST 42, and the expression was induced by IPTG in E.coli. Results: A high level expression was obtained by 0.4mmol/L IPTG inducement in recombined adiponectin E.coli. Conclusion: Human adiponectin gene in E.coli is successfully cloned and expressed.