Construction of Prokaryoti PQE30/Adiponectin Expression Vector and Its Expression in Escherichia Coli

Objective:To construct the PQE30/adiponectin(ADPN) vector and identify its expression in Escherichia(E.) coli.Methods:The encoding fragment of human ADPN gene was obtained from a recombinant plasmid PUC57/ADPN.Using enzymes to digest plasmids,the encoding fragment of human ADPN gene was ligated into expression vector PQE30.After transformation,it was introduced into E.coli JM109 competent cells.After choosing and sequencing,the recombinant vector PQE30/ADPN was constructed,and its expression was induced by IPTG.Results:A 753 bp fragment was obtained by PCR clone.After blotting and sequencing,the results were matched to the cDNA of human ADPN,which accession of the GenBank was NM-004797.An expression was obtained by 0.4 mmol/L IPTG inducement in E.coli M15.Conclusion:The prokaryoti PQE30/ADPN vector was first successfully constructed and expressed.