STUDY ON DRUG-RESISTANCE TO SEVEN ANTICOCCIDIA DRUGS OF CHICKEN COCCIDIAN FROM QINGYUAN, GUANGDONG

We collected the excrement from the broiler farm in Qingyuan, Guangdong and isolated, propagated the coccicial oocysts in the test in order to know drug resistant degree of coccidia and make a proper program for scientific pharmaceuticals. Among the isolated occysts, the total percent of E.acervulina and E.mivati was 81.3% , E.tenella was 18.7%. We selected seven drugs to study the drug resistance of chicken coccidia in the test. The drugs included Diclazuril, Maduramicin, Monensin, Lasalocid, Amprolian, Hainanmycin and Robenidine. The experiment contained 7 medicated groups, 1 inoculated unmedicated and 1 uninoculated unmedicated controls group. There were 10 birds in every group. On fifteen day old, all groups were inoculated with 12×10 4 sporulated oocysts per bird. The criteria used to measure the sensitivity of Eimeria species included anticoccidial index(ACI), reduction of lesion score(RLS), relative oocysts production(ROP) and percent of optimum anticoccidial activity(POAA). There results indicated the isolated strain was light resistance to Diclazuril, partly resistance to Hainanmycin、 Robenidine, and complete resistance to Maduramicin、 Monensin、 Lasalocid、 Amprolian.