Pharmacokinetics and plasma concentration of gatifloxacin in healthy voluntees

OBJECTIVE To determine the plasma concentration and pharmacokinetics of gatifloxacin in healthy voluntees.METHODS The plasma concentration of gatifloxacin was determined by HPLC with the detection wavelength of 293 nm . Its pharmacokinetics was studied after oral administration of a single dose of 400mg to healthy voluntees.RESULTS The linear range was 0.057 ～ 5.22 mg ·L -1 , recovery was between 97.12% ～ 105.56% ,within day and between day RSD was less than 15%.The drug time curve after oral dose of 400 mg gatifloxacin in 10 male voluntees fitted to a two conpartment model.The main pharmacokinetic parameters of gatifloxacin were as follows: T max was 1.97 h ; C max was 3.01 mg ·L -1 ; t 1/2(β) was 9.83 h ;AUC was 28.77 mg ·h·L -1 .CONCLUSIONS The HPLC we developed for gatifloxacin determination is simple and is suitable for plasma drug concentration monitoring and human pharmacokinetic research.