Effect on expression of caspase-3 in the apoptosis of cultured human umbilical Vascular Smooth Muscle cells induced by Proteasome Inhibitor MG132

Objective To study the effect of proteasome inhibitor MG132 on expression of caspase3 and apoptosis in cultured human umbilical vascular smooth muscle cells.Methods human umbilical vascular smooth muscle cells were treated with MG132(2.5μmol·L-1, 5μmol·L-1, 10μmol·L-1) for 48 hours.The apoptotic cells were determined by DNA fragment analysis and flow cytometric analysis.The level of caspase3 mRNA expression were detected by reverse transcription-polymerase chain reaction(RT-PCR).Caspase3 protein expression was detected by immuno-cytochemistry.Results The rate of apoptosis as increased obviously after treatment with MG132 for 48 hours.RT-PCR showed up-regulated gene/protein expression of caspase3.Conclusions Proteasome inhibitor MG132 could induce human umbilical vascular smooth muscle cells in a dose-dependent manner;Possibly through MG132 inhibition on upp activity which promotes caspase3 gene transcription and thereby leads to increased production of intracellular caspase3 protein.