Determination of the Content of Gallic Acid in Huai Chrysanthemum by HPLC

To established HPLC determination method of gallic acid content in huai Chrysanthemum,Agilent C18 column(150 mm × 4.6 mm,5 μm),V(methanol)+V(ultra-pure water +0.1% phosphoric acid)=25+75 as mobile phase,flow rate: 0.5 mL·min-1;column temperature was 25 ℃,detection wavelength: 258 nm;injection volume: 10 μL.The results showed that the regression equation was(y = 3.054 2 x-2.207 1,r2 = 0.999 1(n = 6),the linear range of 0 ~ 20 μg,the average recovery rate of 97.28%,RSD 3.39%.Samples of gallic acid content of 3.17 mg/g.It concludes the method is rapid,simple,accurate,reproducible,reliable,and can be used as arms of gallic acid in chrysanthemum the basis for quality evaluation.