The Continuously Stirring Culture of Human Hepatocytes

Objective To explore a method for cultivation of human hepatocytes in high viability for longer Methods With the condition of the cell restricted to attached dish,culture of human hepatocytes was carried out by continuously gentle stirring in hormonally defined media. The cell yield and amount of albumin synthesis were determined,and the cell growth was inspected under a light microscope and electronmicroscope at regular interval. Results Human hepatocytes could produce to a certain extent. The cell density could maintain up 4.0×10 5/ml from the day 2 to 14 after cultivating. The human hepatocytes could retain the ability to secret albumin up to 20th day. All these were obviously better than the controls. Multicellular aggregates could keep aggregatoinal characteristics to 20th day by light microscope,and on the 15th day,most of that have normal super\|microstructure characteristics of hepatocyte by electron microscope. Conclusion The method could retain human hepatocyte high viability for a longer time. So it was a significant probe for the cell cultivation and application of extracorporeal bioartificial liver support systems.\;