Simple and high efficacy culture and identify of mouse hepatocytes

Objective To search for an easier and higher efficiency method for the primary culture of murine hepatocytes,and identify it.Methods The method of tissue piece adherence was improved for the primary culture.The harvested hepatocytes were kept in a humidified incubator with a little medium about 1～2 h.The serial subcultivation of murine monolayer hepatocytes was performed in a simple way,in which hepatocytes were digested by 0.25% trypsin and cultured with calf serum medium.The whole procedure did not require centrifugation.Results The higher purity hepatocytes and its passage cells was successfully obtained.For further identifing this method,the primary culture of murine hepatocytes was performed all successfully many times.Conclusion The method for the primary culture of murine hepatocytes is a good and economic method for the general lab to culture the hepatocytes.