2004•PubMedRequires access

[Immunoscreening of newborn larvae cDNA library of Trichinella spiralis].

Xiuping Wu, Bao‐Quan Fu, Mingyuan Liu, Yalan Zhang, Lihong Yuan, Lianrui Li, Qiang Lu, Pascal Boireau

Abstract

OBJECTIVE: To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis. METHODS: Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed. RESULTS: 158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis. CONCLUSION: Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.

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Key concepts: Immunoscreening, Trichinella spiralis, Biology, Trichinella, cDNA library, Complementary DNA, Antigen, Recombinant DNA