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Immunoscreening and Analysis of Muscle Larvae cDNA Library of Trichinella spiralis

Bao‐Quan Fu, Yuan Li-hong, Umr I

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Abstract

Muscle larvae (ML) cDNA library of Trichinella spiralis was screened using swine serum infected with Trichinella spiralis. From 1.6×10~5 recombinant 21 positive clones were obtained. Sequence analysis showed that 9 of them were new genes, 9 of them without ORF showed high homology to T.spiralis mitochondrion genome and encoded large subunit ribosomal RNA, 3 of them encoded the known genes. Clones ML12 and 34 showed 99% homology to serine protease inhibitor of T.spiralis(AAF63473)and clone ML42 showed 99% and 90% homology to hypothetical ORF 17.20 of T.spiralis(AAB48489)and 21 kD Excretory/secretory protein of T.pseudospiralis (AAF79206), respectively. These results lay the foundation for further study of the recombinant antigen.

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What this paper is about

Muscle larvae (ML) cDNA library of Trichinella spiralis was screened using swine serum infected with Trichinella spiralis. From 1.6×10~5 recombinant 21 positive clones were obtained. Sequence analysis showed that 9 of them were new genes, 9 of them without ORF showed high homology to T.spiralis mitochondrion genome and encoded large subunit ribosomal RNA, 3 of them encoded the known genes. Clones ML12 and 34 showed 99% homology to serine protease inhibitor of T.spiralis(AAF63473)and clone ML42 showed 99% and 90% homology to hypothetical ORF 17.20 of T.spiralis(AAB48489)and 21 kD Excretory/secretory protein of T.pseudospiralis (AAF79206), respectively. These results lay the foundation for further study of the recombinant antigen.

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Available abstract

Muscle larvae (ML) cDNA library of Trichinella spiralis was screened using swine serum infected with Trichinella spiralis. From 1.6×10~5 recombinant 21 positive clones were obtained. Sequence analysis showed that 9 of them were new genes, 9 of them without ORF showed high homology to T.spiralis mitochondrion genome and encoded large subunit ribosomal RNA, 3 of them encoded the known genes. Clones ML12 and 34 showed 99% homology to serine protease inhibitor of T.spiralis(AAF63473)and clone ML42 showed 99% and 90% homology to hypothetical ORF 17.20 of T.spiralis(AAB48489)and 21 kD Excretory/secretory protein of T.pseudospiralis (AAF79206), respectively. These results lay the foundation for further study of the recombinant antigen.

Key concepts: Trichinella spiralis, Immunoscreening, Biology, cDNA library, Molecular biology, Complementary DNA, Recombinant DNA, Homology (biology)

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Immunoscreening and Analysis of Muscle Larvae cDNA Library of Trichinella spiralis — Research Paper | ScholarLens