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2014•Liaoning Zhongyiyao Daxue xuebaoRequires access

Study on Quality Standard of Chenzhu Oral Liquid

BI Xiao-l

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Abstract

Objective:To establish the quality standard of Chenzhu Oral Liquid. Methods:TLC was used for the qualitative identification of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen. HPLC was used to determine the contents of hesperidin. The samples were separated on the Discovery? HS C18 column;the mobile phase was acetonitrile-0.05% trifluoroacetic acid(20∶80)and the detection wavelength was set at 280 nm. Results:The TLC spots of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen were clear and well-separated without interference of negative samples. The linear relationship of hesperidin was good at the range of 0.0431~0.4310 μg(r=0.9999);the average recovery rate was 101.38%,RSD=1.08%. Conclusion:The method is simple,accurate and reliable with good specificity,which can be used for the quality control of Chenzhu Oral Liquid.

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Objective:To establish the quality standard of Chenzhu Oral Liquid. Methods:TLC was used for the qualitative identification of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen. HPLC was used to determine the contents of hesperidin. The samples were separated on the Discovery? HS C18 column;the mobile phase was acetonitrile-0.05% trifluoroacetic acid(20∶80)and the detection wavelength was set at 280 nm. Results:The TLC spots of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen were clear and well-separated without interference of negative samples. The linear relationship of hesperidin was good at the range of 0.0431~0.4310 μg(r=0.9999);the average recovery rate was 101.38%,RSD=1.08%. Conclusion:The method is simple,accurate and reliable with good specificity,which can be used for the quality control of Chenzhu Oral Liquid.

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Available abstract

Objective:To establish the quality standard of Chenzhu Oral Liquid. Methods:TLC was used for the qualitative identification of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen. HPLC was used to determine the contents of hesperidin. The samples were separated on the Discovery? HS C18 column;the mobile phase was acetonitrile-0.05% trifluoroacetic acid(20∶80)and the detection wavelength was set at 280 nm. Results:The TLC spots of Citri Reticulatae Pericarpium,Rhizoma Atractylodis Macrocephalae and Coicis Semen were clear and well-separated without interference of negative samples. The linear relationship of hesperidin was good at the range of 0.0431~0.4310 μg(r=0.9999);the average recovery rate was 101.38%,RSD=1.08%. Conclusion:The method is simple,accurate and reliable with good specificity,which can be used for the quality control of Chenzhu Oral Liquid.

Key concepts: Quality standard, Hesperidin, Medicine, Chromatography, High-performance liquid chromatography, Traditional medicine, Chemistry, Pathology

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