Construction of P-selectin lectin-like domain fused with green fluorescent protein gene

Objective To construct a fused gene of P selectin lectin like domain and green fluorescent protein(pEGFP N1/L) to provide the scientific basis to clarify the pathological physiology sense of P selectin. It also has laid a foundation to analyze the relationship between molecular structure and function. Methods The lectin like region fragment of P selectin obtained by PCR was insert into pEGFP N1. The fusion gene was verified by enzyme digestive analysis and sequencing. Results A positive band was formed when product of PCR electrophoresis on agarose gel and insert fragment was confirmed correctly by enzyme digestion analysis and sequencing. Conclusion The fusion gene pEGFP N1/L was constructed successfully by genetic engineer technique. It has laid a foundation to study P selectin's structure function relationship by GFP used as biology marker molecular.