Influence of YunnanBaiyao on the stimulating effects of platelet-rich plasma on proliferation and differentiation of rabbits bone marrow stromal stem cells

AIM:To investigate whether YunnanBaiyao affects the stimulating effects of platelet-rich plasma (PRP) on the proliferation and differentiation of rabbit bone marrow stromal stem cells (rBMSCs). METHODS: rBMSCs were isolated from 4-month-old New Zealand rabbit bone marrow and selected by the adherent method. Flow cytometry was used to confirm the identification of rBMSCs. The 3rd passage rBMSCs were plated at 5×104 /well in 96 well plates in L-DMEM. Cells were treated with PRP plus various concentrations of Yunnanbaiyao. Cells without treatment and with PRP treatment only served as controls. The proliferation of rBMSCs was examined by MTT method and the alkaline phosphatase (ALP) activity was determined by enzyme assay on day 3, 5 and 7. RESULTS: At concentration of 60 μg/mL and 65 μg/mL Yunnanbaiyao showed statistically significant enhancing effects on PRP- stimulated rBMSCs proliferation at all time points (P0.05). At concentrations of 60, 65, 70 and 75 μg/mL Yunnanbaiyao greatly enhanced the stimulating effects of PRP on rBMSCs ALP levels on day 5 and day 7 (P0.05). However, no significant difference was observed between the PRP and PRP+ Yunnanbaiyao group in ALP activity on day 3 (P0.05). CONCLUSION: YunnanBaiyao can promote the stimulatory effects of PRP on the proliferation and differentiation of rBMSCs.