Research on Callus Induction and Regeneration from Mature Embryos of Maize

【Objective】The purpose of this research was to study the tissue culture conditions of corn matured embryo and affecting factors of plant regeneration frequency to establish high-performance regeneration system of corn matured embryo.【Method】The effects of correlation affecting factors on the eallus induction of matured embryo explant,successive and differentiation cultiveation were studied by use of corn matured embryo as test materials.【Result】2.4-D concentrations should be in 1.0-2.0 mg/L between olumes concentration of 0.4-0.5 mg/L range significantly promote the radicle callus to grow.6-BA concentration 0.1-0.02 mg/L were suitable for callus culture.The growth quality of callus of Xinzi 523 were the best in three inbreds stirains.And it is easy to produce sexual embryo callus,but there were just few callus in Han 21.The differentiation capacity of Han21 and Xinzi 523 were identical with each other,under 0.5 mg/L NAA,1.5 mg/L 6-BA,100 mg/L AgNO3,and both are easier to take root,regeneration seedling frequency reaches 30%-40%,however the differentiation rate and rooting ability of B73 are relatively weak.【Conclusion】The callus produced by the induction of corn matured embryo,differentiation and produced explants were the favorable supplement for young embryo regeneration system.