Effect of B(a)P on Lipid Peroxidation Levels in Coke Oven Workers and Rats

[Objective] To explore effect of B(a)P on levels of lipid peroxidation in coke oven workers and rats. [Methods] 134 coke oven workers and 36 controls were chosen and received questionnaires; and the activity of total superoxidedismutases (T-SOD), glutathione(GSH)and malondialdehyde(MDA)were determined. 40 healthy adult SD rats were divided into 4 groups at random: normal oil control, B(a)P 1.3 mg/kg, B(a)P 3.2 mg/kg and B(a)P 7.8 mg/kg. B(a)P was intraperitioneal injected every 2 days for 60 d. After exposure, enzymes(SOD, GSH, MDA, ROS)in blood and hippocampus cell mitochondrion were detected by test kits. [Results] Compared with the controls, lower levels of SOD, GSH were observed in the workers; while significandy higher MDA levels were found in workers than those in controls. In mice blood, compared with the control group, the activity of SOD was increased in B(a)P 7.8 mg/kg group(P0.05), the levels of ROS were lower in B(a)P 7.8 mg/kg and B(a)P 3.2 mg/kg group (P0.05), but no significant change was found in MDA level(P0.05). Compared with the control group, in hippocampus cells, the levels of SOD and GSH were significantly lower in B(a)P 7.8 mg/kg group(P0.05), and MDA level was higher(P0.05). The level of GSH in B(a)P 3.2 mg/kg group were significantly decreased compared with the control group (P0.05). [Conclusion] It suggested that polycyclic aromatic hydrocarbons could induce biomembrane injury, and had more serious effect on brain.