The protective effects of captopril on apoptosis of human umbilical vein endothelial cells under high concentrations of glucose in vitro

The human umbilical vein endothelial cells(HUVEC) were cultured in five groups:NG(Glu 5.5 mmol/L,normal control),HG20(Glu 20 mmol/L),HG40(Glu 40 mmol/L),HG20-Cap(Glu 20 mmol/L,Cap10-5 mol/L) and HG40-Cap(Glu 40 mmol/L,Cap10-5 mol/L).Apoptosis of the cells and the expressions of Fas,Bcl-2 were determined and evaluated by flow cytometry.Exposure to HG resulted in an increase in apoptosis in both HG20 and HG40 groups(P0.01).HG enhanced the expression of Fas and decreased Bcl-2 expression.Captopril reduced high-glucose-induced HUVEC apoptosis accompanied by a decrease of Fas expression and an increase of Bcl-2 expression.