Protective Effect of Benazepril Hydrochloride on the Injury of Human Umbilical Vascular Endothelial Cells Induced by High Glucose

Objective To investigate the protective effect of benazepril hydrochloride on the injury of human umbilical vein endothelial cells induced by high glucose in vitro,and to explore its underlying mechanism.Methods Cultured human umbilical vein endothelial cells(HUVEC)were respectively cultured with 5.5mmol/L glucose(Glu)(group A),33.3mmol/L Glu(group B),33.3mmol/L Glu+0.1μmol/L hydrochloride(Ben)(group C),33.3mmol/L Glu+1μmol/L Ben(group D),33.3mmol/L Glu+10μmol/L Ben(group E)for 24h.The morph sis of cell was observed by inverted phase contrast microscope;and the cell vitality as well as the activity of GSH-PX,MDA content,and the secretary content of nitric oxide(NO)in supernatant were measured.Results In the group C,D and E the cell vitality,activity of GSH-PX and MDA content were significantly different with those in group B(P0.01).but the secretary content of NO shed no significant difference(P0.05).Conclusion Benazepril hydrochloride can protect the injury of HUVEC induced by high glucose in vitro,and the mechanism may be associated with scavenging reactive oxygen radicals,reducing lipid peroxidation,and increasing the activities of intracellular antioxidase system.