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Construction of an eukaryotic expression vector inserted human IκBα mutant gene

Yong Zhang

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Abstract

Objective: To construct a recombinant eukaryotice expression plasmid inserted human(IκBα) mutant gene. Methods: RTPCR and overlap extension PCR were used to get site-mutant human IκBα.The RCR product was cloned into plasmid PcDNA 3.0.The accuracy of PcDNA3.0-IκBαM was confirmed by restriction enzyme digestion and DNA sequencing. Results: Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted IκBαM gene(PcDNA3.0IκBαM) had been constructed correctly. Conclusion: An eukaryotice expression plasmid PcDNA3.0-IκBαM has been constructed successfully.

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What this paper is about

Objective: To construct a recombinant eukaryotice expression plasmid inserted human(IκBα) mutant gene. Methods: RTPCR and overlap extension PCR were used to get site-mutant human IκBα.The RCR product was cloned into plasmid PcDNA 3.0.The accuracy of PcDNA3.0-IκBαM was confirmed by restriction enzyme digestion and DNA sequencing. Results: Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted IκBαM gene(PcDNA3.0IκBαM) had been constructed correctly. Conclusion: An eukaryotice expression plasmid PcDNA3.0-IκBαM has been constructed successfully.

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Available abstract

Objective: To construct a recombinant eukaryotice expression plasmid inserted human(IκBα) mutant gene. Methods: RTPCR and overlap extension PCR were used to get site-mutant human IκBα.The RCR product was cloned into plasmid PcDNA 3.0.The accuracy of PcDNA3.0-IκBαM was confirmed by restriction enzyme digestion and DNA sequencing. Results: Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted IκBαM gene(PcDNA3.0IκBαM) had been constructed correctly. Conclusion: An eukaryotice expression plasmid PcDNA3.0-IκBαM has been constructed successfully.

Key concepts: Overlap extension polymerase chain reaction, Plasmid, Recombinant DNA, Mutant, Restriction enzyme, Molecular biology, Gene, Biology

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