ScholarLens Back to search
2011•China Animal Husbandry & Veterinary MedicineRequires access

Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody

Qi-Lin Meng

Open publisher page 0 citations

Abstract

In order to prepare the monoclonal antibody of goose parvovirus,BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells(4B4) were obtained by hybridoma technique,indirect ELISA and limiting dilution assay.After identification,the heavy chain of McAb was IgG1 subtype and the light chain was κ chain.The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb.It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein.This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent.

About this research paper

What this paper is about

In order to prepare the monoclonal antibody of goose parvovirus,BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells(4B4) were obtained by hybridoma technique,indirect ELISA and limiting dilution assay.After identification,the heavy chain of McAb was IgG1 subtype and the light chain was κ chain.The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb.It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein.This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

In order to prepare the monoclonal antibody of goose parvovirus,BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells(4B4) were obtained by hybridoma technique,indirect ELISA and limiting dilution assay.After identification,the heavy chain of McAb was IgG1 subtype and the light chain was κ chain.The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb.It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein.This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent.

Key concepts: Monoclonal antibody, Epitope, Molecular biology, Goose, Antibody, Heavy chain, Virology, Blot

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody — Research Paper | ScholarLens