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2011•Progress in Veterinary MedicineRequires access

Preparation of Monoclonal Antibody Against VP3 Protein of Goose Parvovirus

Fang Jing-tian

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Abstract

Balb/c mice were immunized with purified goose parvovirus(GPV) VP3 to prepare the monoclonal antibody.The anti-GPV hybridoma cells(4E5)were prepared with hybridoma technique,indirect ELISA and limiting dilution assay for 3 times were used to obtain the monoclonal antibody hybridoma cells.After identification,the MAb is IgG1 subtype and the light chain is κ.The results of Western blot showed that GPV VP3 protein could be specially recognized by 4E5 MAb.The anti-GPV VP3 monoclonal antibody was prepared successfully,which lay a foundation for diagnosis and treatment of GPV.

About this research paper

What this paper is about

Balb/c mice were immunized with purified goose parvovirus(GPV) VP3 to prepare the monoclonal antibody.The anti-GPV hybridoma cells(4E5)were prepared with hybridoma technique,indirect ELISA and limiting dilution assay for 3 times were used to obtain the monoclonal antibody hybridoma cells.After identification,the MAb is IgG1 subtype and the light chain is κ.The results of Western blot showed that GPV VP3 protein could be specially recognized by 4E5 MAb.The anti-GPV VP3 monoclonal antibody was prepared successfully,which lay a foundation for diagnosis and treatment of GPV.

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Available abstract

Balb/c mice were immunized with purified goose parvovirus(GPV) VP3 to prepare the monoclonal antibody.The anti-GPV hybridoma cells(4E5)were prepared with hybridoma technique,indirect ELISA and limiting dilution assay for 3 times were used to obtain the monoclonal antibody hybridoma cells.After identification,the MAb is IgG1 subtype and the light chain is κ.The results of Western blot showed that GPV VP3 protein could be specially recognized by 4E5 MAb.The anti-GPV VP3 monoclonal antibody was prepared successfully,which lay a foundation for diagnosis and treatment of GPV.

Key concepts: Monoclonal antibody, Molecular biology, Western blot, Antibody, Goose, Virology, Hybridoma technology, Monoclonal

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