Isolation,Identification and Quantitative Analysis of Matrine and Oxymatrine in Sophora tonkinensis Gagnep. var. polyphylla

Objective To study the isolation and assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep. var. Polyphylla. Methods The chemical constituents were extracted by solvent and then isolated by silica gelchromatographycolumn. The chromatographic technique was used to identify its chemical structures. The chromatographic conditions of assaying by high performance liquid chromatography( HPLC) as follows: Kromasil 100-5 NH2column( 250 mm × 4. 6 mm,5 μm) with acetonitrile-monometallic sodium orthophosphate( 0. 05 mol/L)-phosphoric acid solution( 3. 0%)-ethylalcohol( 80 ∶ 5 ∶ 9. 5 ∶ 5. 5) as mobile phase,the flow rate was 1. 0 ml /min,the UV detection wave length was 210 nm,and the column temperature was 30℃. Results The two alkaloids compounds attained from the separation of Sophora tonkinensis Gagnep. var. Polyphylla were identified as matrine and oxymatrine. The matrine formed linear relation with its peak area when it was in the range of 0. 0164- 0. 1476 μg. The oxymatrine formed linear relation with its peak area when it was in the range of 0. 2872- 2. 5848 μg. The average recovery rates of matrine and oxymatrine were 100. 2%( RSD = 0. 76%) and 99. 8%( RSD = 1. 19%),respectively. Conclusion Matrine and oxymatrine are attained from the separation of Sophora tonkinensis Gagnep. var. Polyphylla for the first time. The established method( HPLC) in this study is simple,reliable,reproducible and accurate. It could be applied to the assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep. var. polyphylla.