Preparation of curcumin liposomes and studies of its anti-lipid peroxidation in vitro

Objective To prepare curcumin liposomes and study its impact on the lipid peroxidation in rat tissues in vitro.Methods Curcumin liposomes were prepared by film dispersion and extrusion technique.Then encapsulation efficiency(EE)of curcumin liposomes was determined by minicolumn centrifugation method.Orthogonal experimental design was used to optimize the formulation.To evaluate the impact of curcumin liposomes on the lipid peroxidation in rat tissues,thiobarbituric acid(TBA)method was adopted to detect the amounts of malondialdehyde(MDA),a product of lipid peroxidation reaction.Results The average EE of curcumin liposomes prepared according to the optimized formulation was(89.32±1.58)%.The inhibition rate of curcumin liposomes on rat hearts,livers,kidneys and brains lipid peroxidation was 70.02%,59.48%,59.10%,85.21%,respectively in vitro.In addition,as the amount of curcumin liposomes increased,the inhibition rate increased accordingly.Conclusion Using film dispersion technique for preparation of curcumin liposomes is simple and feasible.Curcumin liposomes can significantly inhibit lipid peroxidation in rat tissues in vitro.