ScholarLens Back to search
2005•Journal of Medical ForumRequires access

Determination of Three Main Components in Belladonna Tablets by RP-HPLC

Zhenzhong Zhang

Open publisher page 0 citations

Abstract

Objective To establish a method for determination of scopolamine , anisodamine and atropine in Belladonna tablets by RP-HPLC.Methods The concentrations were quantified by external standard method and RP-HPLC. Results The calibration curve for atropine , anisodamine and scopolamine revealed linearity over the range of 7.55~37.75 μg/ml , 2.32~20.88 μg/ml and 2.5~12.5 μg/ml .The average recoveries of atropine, scopolamine and anisodamine were 100.5%(n=3,RSD=1.3 %) ,99.3%(n=3,RSD=1.6 %),99.9%(n=3,RSD=1.4%) respectively. Conclusion The method is successfully used to rapid assay belladonna teblet with accurate,sensitive, reproducible and little sample needed.

About this research paper

What this paper is about

Objective To establish a method for determination of scopolamine , anisodamine and atropine in Belladonna tablets by RP-HPLC.Methods The concentrations were quantified by external standard method and RP-HPLC. Results The calibration curve for atropine , anisodamine and scopolamine revealed linearity over the range of 7.55~37.75 μg/ml , 2.32~20.88 μg/ml and 2.5~12.5 μg/ml .The average recoveries of atropine, scopolamine and anisodamine were 100.5%(n=3,RSD=1.3 %) ,99.3%(n=3,RSD=1.6 %),99.9%(n=3,RSD=1.4%) respectively. Conclusion The method is successfully used to rapid assay belladonna teblet with accurate,sensitive, reproducible and little sample needed.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish a method for determination of scopolamine , anisodamine and atropine in Belladonna tablets by RP-HPLC.Methods The concentrations were quantified by external standard method and RP-HPLC. Results The calibration curve for atropine , anisodamine and scopolamine revealed linearity over the range of 7.55~37.75 μg/ml , 2.32~20.88 μg/ml and 2.5~12.5 μg/ml .The average recoveries of atropine, scopolamine and anisodamine were 100.5%(n=3,RSD=1.3 %) ,99.3%(n=3,RSD=1.6 %),99.9%(n=3,RSD=1.4%) respectively. Conclusion The method is successfully used to rapid assay belladonna teblet with accurate,sensitive, reproducible and little sample needed.

Key concepts: Anisodamine, Chromatography, Scopolamine, High-performance liquid chromatography, Atropine, Chemistry, Calibration curve, Atropa belladonna

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Three Main Components in Belladonna Tablets by RP-HPLC — Research Paper | ScholarLens