AFLP Fingerprinting Analysis of Different Pear Cultivars

Eight pairs of AFLP primers were screened out and used for the analysis of 30 Pyrus cultivars such as Aiganshuili pyrus et al.These primers generated 203 fragments,135 polymorphism fragments among them.Percentage of the polymorphism fragments produced by each pair of primers was 66.5% in average.All these data indicated that considerable genetic variation existed among the studied pear cultivars at DNA level.Molecular genetic distances among the studied cultivars were calculated,and the relationship among them was described quantitatively.All of the cultivars were clustered into 4 groups.The cultivars of the 1st group were the Japanese pear or have genetic relationship with Japanese pear.The cultivars of the 2nd group were mainly the cultivars of Chinese pear.Huangguanli pyrus and Qingxiangli pyrus were clustered to the 3rd group;these two cultivars had a common parent of Shinseiki pyrus.The 4th group had only 1 cultivar of Kangdeli pyrus.One of its parents was Pyrus communis,was clustered into 1 group.The clustering resutls showed that different varieties had certain regional corretotion.