Effect of Guanxinshutong on cerebral ischemia reperfusion in rats learning and memory ability and brain Bcl-2,Bax protein expression

Objective: Guanxinshutong on cerebral ischemia reperfusion( I/R) in rats learning and memory ability and brain Bcl-2,Bax protein expression to explore the protection mechanisms of Guanxinshutong on cerebral I /R injury in brain tissue. Methods:Healthy Wistar rats for the study were randomly divided into sham operation group,I /R model group,high dose Guanxinshutong( 1 g /kg) and low dose Guanxinshutong( 0. 5 g /kg) group,n = 15. cerebral I /R model was estabhished by using the left commom cartid artery ligation method,modeling each group were fed daily before saline,saline,Guanxinshutong( 1 g /kg) and Guanxinshutong( 0. 5 g /kg),continuous 7 days; 3 d after water maze training while continuing administration,continuous 7 d,the eight day,Morris water maze test;after the test,the rats were sacrificed blood and brain tissue,serum by ELISA of Bcl-2 and Bax protein levels change; using HE staining of rat brain pathology; using immunohistochemistry assay in rat brain organization of Bcl-2 and Bax protein levels changes. Results:Compared with the sham group,Guanxinshutong high-dose group test latency slightly longer timers after platform and platform quadrant dwell decreased slightly,serum and brain tissue slightly elevated levels of Bax protein,Bcl-2 protein levels decreased slightly but not significantly different( P0. 05),low-dose group Guanxinshutong through latency test in rats,after the number of platforms and platform quadrant dwell time decreased,serum and brain tissue elevated levels of Bax protein,Bcl-2 protein content decreased significantly different( P 0. 01),cerebral I /R model group was significantly prolonged latency test,after the number of platforms and platform quadrant dwell time significantly reduced serum and brain tissue was significantly increased Bax protein,Bcl-2 protein content decreased with significant differences( P 0. 01). Conclusion: Guanxinshutong can significantly improve spatial memory,and by reducing Baxprotein content and increased Bcl-2 protein,inhibition of cerebral I /R injury of apoptosis,and thus the brain tissue I /R has a protective effect.