Succinic acid fermentation in different medium conditions by recombinant Escherichia coli

The production of succinic acid by bacterial fermentation has a great prospect for industrial application. In this study,the fermentative productions of succinic acid by recombinant Escherichia coli WS100(△ldhA,△adhE,△pflB,△poxB,△ackA) with different media were investigated. 4L NBS medium and corn steep liquor medium were utilized respectively for fed batch fermentations with E.coli WS100 in fermenters. After 83h of fermentation,96.17g/L glucose was consumed in corn steep liquor medium,with the production of 63.45g/L succinate. The productivity and yield reached 0.76g/(L·h) and 65.98%,respectively. In NBS medium,110.30g/L glucose was consumed with the production of 84.98g/L succinate. The productivity and yield reached 1.02g/(L·h) and 77.04%,respectively. The results indicated fine succinate fermentative production was by WS100 in both media,and with higher productive amount,productivity and yield in NBS medium.