HPLC Determination of 6-Benzoylcaproic Acid
Han Xue-mei
Abstract
Han Xue-mei
Abstract
Determination of 6-benzoylcaproic acid by HPLC was reported.Kromail-C18 column(4.6mm×220mm,5μm) was used,with the flow rate of 0.8 mL/min,the mobile phase of methanol-1% of H3PO4(pH 4.5,adjusted with TEA)(70:30) and the detection wavelength of 242 nm.Result showed that the linear range was 0.1 ~0.7 mg/mL,with r of 0.999 97 and RSD of 0.46%.The method was simple,accurate and reliable.
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Determination of 6-benzoylcaproic acid by HPLC was reported.Kromail-C18 column(4.6mm×220mm,5μm) was used,with the flow rate of 0.8 mL/min,the mobile phase of methanol-1% of H3PO4(pH 4.5,adjusted with TEA)(70:30) and the detection wavelength of 242 nm.Result showed that the linear range was 0.1 ~0.7 mg/mL,with r of 0.999 97 and RSD of 0.46%.The method was simple,accurate and reliable.
Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Methanol, Linear relationship, Volumetric flow rate, Detection limit