Influence of Daunorubicin on Nuclear Factor KappaBp65 Activity of K562 Cells

Objective To explore the influence of daunorubicin on nuclear factor kappaB (NF-κB)p65 activity and apoptosis of K562 cells.Methods The K562 cell was divided into control group:the K562 cells were respectively cultivated with RPMI-1640; daunorubicin(DNA) group:the K562 cell were treated respectively with respectively of DNR 0.2 μmol/L,2.0 μmol/L and 20.0 μmol/L.The activation of NF-κBp65 of K562 cell was determined by immuno-histological staining,after respectively treated for 1,2,6 and 24 h.Results After treated for 1,2,6 h,the rates of activation of NF-κBP65 of K562 cells in DNA group had no significant difference compared with control group (Pa0.05),and 24 h with DNR 0.2 μmol/L,its positive rate was significantly higher compared with control group(P0.001). After treated for 1 h with DNR 2.0 μmol/L,there was no significant difference compared with control group(P0.05),and after treated for 2,6 and 24 h,DNR 2.0 μmol/L,there was significant difference compared with control group (Pa0.001). Treated respectively for 1,2,6 and 24 h with DNR 20.0 μmmol/L,their positive rates were obvious difference compared with control group(Pa0.001).Conclusion DNR can induce the apoptosis of K562 cells which promoting the activation of its NF-κBp65,dose-dependence and time-dependence exist for the effect of DNR.