Influence of daunorubicin on apoptosis factor of K562 cells

Objective To explore the influence of daunorubicin on apoptosis factor of K562 cells. Methods The K562 cell was divided into control group and DNA group.Control group:the K562 cells were respectively cultivated with RPMI-1640;daunombiein(DNA)group:the K562 cells were treated respectively with respectively of DNR 0.2,2 and 20 μmol/L.The apoptosis factor of K562 cells was determined by FCM,after respectively treated for 1,2,6 and 24 h.Results K562 cells existed natural apoptosis without durg treatment.After treated for different time with DNA 0.2 μmol/L,the rates of apoptosis factor of K562 cells in DNA group had no significant difference compared with control group(P0.05).After treated for 1 h with DNA 2 μmol/L,there was no significant difference compared to the contrast group(P0.05).After treated for 2,6 and 24 h,DNA 2 μmol/L,there was significant difference compared with the control group(P0.01).Treatment for 1,2,6 and 24 h with DNA 20 μmol/L,the apoptosis rates were obviously difference compared with control group(P0.01).Conclusion DNR can induce the apoptosis of K562 cell,which existed dose-dependence and time-dependence for the effect of DNR.