2012•Shandi Nongye Shengwu xuebaoRequires access
Effects of Agrobacterium Rhizogene Strains and Tobacco Uarieties on the Transformation Rate of Hairy Root
Jiang Long
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Abstract
Different Agrobacterium rhizogenes strains and tobacco cultivars on the transformation of hairy root were investigated by 3×3 factor design experiments.The PCR technique was employed to detect hairy root.The results showed that rolB gene in Ri plasmid of A.rhizogenes strains were integrated into the genome of cell of tobacco and the hairy roots were induced successfully.Different rhizogenes strains and tobacco cultivars influenced the transformation rate of hairy root.The average transformation rate of Sumxun(up to 53.4%) was the highest among the tested cultivars,whith suitable bacterial strains A4 and ATCC15834.The transformation rate with strain R1000 of cultivar K326 was 34.2%,and that of cultivarYunyan85 was 25.6% with strain ATCC15834.The results might provide an experimental basement for the induction of tobacco hairy roots via transformation.