FACTORS AFFECTING THE TRANSFORMATION OF ARTEMISIA ANNUA L. WITH AGROBACTERIUM RHIZOGENES

Factors affecting Artemisia annua L. transformation with Agrobacterium rhizogenes using leaf disc method were studied. The results indicated that the effects of A. rhizogenes strains, basal media and A. annua L. strains on hairy root formation were notable, while that of medium pH ranging between 5.2～5.8 was not. Among the 5 A. rhizogenes strains used, ATCC15834 was the best; MS medium gave the best results and A. annua L. strain 025 was the most sensitive one among the 3 strains tested. After diluted for 5 times, the A. rhizogenes suspension at logarithmic phase was the most suitable preparations for the transformation. The hairy root formation frequency of immatural leaves was higher than that of matural leaves. The addition of acetosyringone to the co culture media had no obvious effects on transformation frequency. A 100% hairy root formation frequency was achieved with A. rhizogenes ATCC15834 at the optimal transformation conditions. The results of PCR amplification with the primers designed for rol C gene indicated that rol C fragment of T DNA of Ri plasmid was keen transferred into the genome of hairy root of A. annua L.