Antithrombotic activity of Ocimum basilicum L. extract and the action of mechanism

AIM:To study the antithrombotic activity of Ocimum basilicum L(OBL) extracts,extracted with aqueous and 70% ethanol,and to observe anticoagulat action and fibrinolysis. METHODS:Three experimetal models in vivo,pulmonary thromboembolism in mice,FeCl3-induced arterial thrombosis and ligation-induced inferior caval thrombosis in rats were used to examine the antithrombotic activity of aqueous and alcohol extract of OBL ; the activity of AT-Ⅲ,PC,PLG,t-PA and PAI-1 in plasma were determined by ELISA to evaluate the effects of thedrugs on the anticoagulant factor and fibrinolytic system. RESULTS: OBL extract by oral administration could markedly increase the survival rate of the mouse suffering from pulmonary thrombosis induced by the mixture of collagen and epinephrine,and significantly decreased the weight of the thrombus induced by ligation of inferior vena cava. The concentration of AT-Ⅲ was decreased,the concentration of PC was increased; the activity of PLG and t-PA were increased,the activity of PAI-1 was decreased slightly,the ratio of t-PA/PAI-1 increased. CONCLU-SION: The OBL extracts,both aqueous and alcholol extract,have strong anti-thrombosis effect,the action of mechanism may have correlation with the increase in the activity of the anticoagulant factor and fibrinolysis system.