2007•Tianjin Yike Daxue xuebaoRequires access
Effect of erythropoietin on expression of Bcl-2 and Bax protein in neonatal rats with HIBD
Chu Bao-feng
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Abstract
Objective: To explore the effects and the molecular mechanisms of exogenous recombinant human erythropoietin (EPO)on the expression of Bcl-2 and Bax protein in brain tissue following hypoxic-ischemic brain damage(HIBD)in newborn rat.Methods: Unsexed 7-day-old Wistar rats were randomly divided into sham operation group,HIBD group,saline-treated control group and EPO-treated group.In the EPO-treated group,pups received different single intraperitoneal injection(10 000 U/kg,5 000 U/kg ,1 000 U/kg) after HIBD.We measured the expression of Bcl-2 and Bax protein at 24 h after injection by immunohistochemical method.Results: The expression of Bcl-2 and Bax protein in HIBD group and saline-treated control group were significantly higher in comparison with that in sham operation group,the Bcl-2/Bax ratio was significantly lower.The expression of Bcl-2 protein and the Bcl-2/Bax ratio in EPO-treated group were significantly higher than those in HIBD group and saline-treated control group,the expression of Bax protein was significantly lower. Conclusion: The neuroprotective mechanism of EPO may be related to up-regulating the expression of Bcl-2 protein,down-regulating the expression of Bax protein,alteration of the Bcl-2/Bax ratio in brain tissue and inhibition of the neuron apoptosis after HIBD.Our conclusion would open a new window for the clinical treatment of neonatal HIBD.