The injuries on HepG2 cells by the plasma of severe hepatitis patients

Objective To observe the injuries of plasma from patients with severe hepatitis on HepG2 cells. For the advanced study on hepatocytes to maintain their function in a bioartificial liver device and improving the clinical efficiency of the bioartificial liver device. Methods HepG2 cells were cultured. Viability, the leakages of LDH, apoptosis and the changes on morphology of HepG2 cells exposured to SHP were measured and investigated. Results ①The viability was inhibited in 10%, 30%, 50%, 100% SHP, a significant difference occurred in the culture medium containing 100% SHP compared to the controls. ②the leakages of LDH was significantly increased in the HepG2 cells cultured with SHP, at the end of the experiment they were approximately 2 times higher than that of HepG2 cells cultured in the medium containing 10% NCS. ③Exposure to SHP,especially the 100% SHP, resulted in a signifiant increase in apoptosis of HepG2 cells compared to control cultures. ④The bullae were observed from SEM pictures of HepG2 cells cultured in SHP for 24h. The higher of the SHP concentration the more dead cells were found. Conclusion Plasma from patients with severe hepatitis can damage HepG2 cells. It could be necessary to pretreat the patients,plasma to increase the efficiency of the bioartificial liver device.