Effects of plasma from patients with chronic severe hepatitis on the proliferation and detoxic capability of HEPG_2 cell line

Aim:To investigate the effects of plasma from patients with chronic severe hepatitis on HEPG2 cell in vitro.Methods:HEPG2 cells were cultured in normal human plasma(NHP) and chronic severe hepatitis plasma(CSHP),respectively.MTT method was used to detect the activity of HEPG2 cell cultured for 24,48,72,96 and 120 h.Cellular morphology was observed by inverted microscope and electron microscope.The content of glutathione(GSH) inside the cells was detected after being cultured for 48 h.Results:The activities of HEPG2 cells cultured in CSHP were higher than those in NHP for successive 5 days(t=5.424,4.714,3.834,3.781,and 6.106,P0.001).HEPG2 cells cultured in CHSP for 24 h looked thicker than that in NHP.The observation through electron microscope demonstrated that the HEPG2 cells cultured for 48 h in both groups grew well,while the cells in prophase of division in CSHP group were much more than that in NHP group.The content of GSH inside HEPG2 cell cultured in CSHP for 48 h was(306.7±16.2) mg/g,lower than that of(492.3±15.7) mg/g in NHP(t=9.18,P0.001).Conclusion:CHSP can not only promote the proliferation of HEPG2 cells,but also decrease the detoxic capability of HEPG2 cells by exhausting GSH inside the cells.