Establishment of a Model with Both Alzheimer's Disease and Spleen Deficiency Syndrome and Empirical Study of Effect of Zi-Bu-Pi-Yin Decoction on It

Objective:In order to correspond to disease with syndrome in clinic more effectively,it is our aim to establish a rat model with both Alzheimer's disease(AD) and spleen deficiency syndrome,then to study the effect of Zi-Bu-Pi-Yin(ZBPY)Decoction on it.Methods:Rats model of spleen Yin deficiency was set up by the classical way.Then the incubated Aβ_1-40 was injected into hippocampus of each rat.The treated group was administered with ZBPY.Observe the changes of the rats at symptom in different phases;the behavioral abnormalities were investigated by step-down test and Morris water maze test.Results:The two model groups of spleen Yin deficiency had the appearance of Yin deficiency leading to endogenous heat,for example that increment in drinking water(P0.01),objective elevation of the body temperature(P0.01).The ability of learning and memory of the two AD groups was reduced conspicuously(P0.05).The symptom of Yin deficiency and abnormal behavior of the ZBPY group were improved in AD rat model(P0.05).Conclusion:Try to perfect the methods to make the models in this experiment and then the changes of behavior and symptom were observed in syndrome,disease,and combined syndrome with disease.ZBPY improved the symptom of spleen Yin deficiency and dysfunction of learning and memory in AD rat combined with Spleen Yin deficiency.