The change of CD5~+B lymphocytes in the peripheral blood of patients with childhood aplastic anemia and its clinical significance

Objective To investigate the quantitative change of CD5+B lymphocytes in the peripheral blood of patients with childhood aplastic anemia and its clinical significance.Methods Quantities of CD5+B lymphocytes in the peripheral blood of 56 patients with childhood aplastic anemia and 60 normal controls were assayed by flow cytometry.The correlation between the therapeutic efficacy of the combined immunotherapy(HDIG+CsA+Prednisone)and the quantities of CD5+B lymphocytes was analyzed.Results The quantity of CD5+B lymphocytes was significantly higher in SAA patients than in normal control;the quantity of CD5+B lymphocytes was significantly higher in effectively cured patients than in ineffective cured patients,and decreased after six months of treatment in effectively cured patients.Conclusion CD5+B lymphocytes in the peripheral blood of patients with childhood SAA increase significantly,which may be related to the onset of the disease.The quantities of CD5+B lymphocytes differ in groups which had different curative effect after being treated by HDIG+CsA+Prednisone,which suggests that CD5+B lymphocytes might be a useful marker to direct the clinical therapy.