2010•Zhongguo shouyi zazhiRequires access
Development of monoclonal antibodies against the P protein of rabies virus.
San-ying Mao, Xiaofeng Guo, He Min, Ma Yongjiang, Fan XiaoLong, Haikuo Yan, Hongna Ma, Deng XianBai
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Abstract
The fusion protein(pET32a(+)-P)was purified by TrapFF crude,6-8-week-old BALB/c mice were immunized with the protein mixed with adjuvants before the splenic cells of immunized mice were fused with SP2/0 myeloma cells.Indirect fluorescence antibody detection assay was used to screen hybridoma cells for the specific monoclonal antibodies.One hybridoma(5G8) producing monoclonal antibody were obtained after three rounds of subcloning.